Say the cells are synchronised in telophase and can we isolate those cells in particular for further procedures? I would like to have a discussion.
Using FACS you can isolate cells in G2/M but sorting out cells in telophase seems quite difficult to me, sorry. Probably you should organise your timing to make coincide the synchronised telophases with your protocol.
Using FACS you can isolate cells in G2/M but sorting out cells in telophase seems quite difficult to me, sorry. Probably you should organise your timing to make coincide the synchronised telophases with your protocol.
I agree with Santiago, you can use to sort your cells of a particular phase.
thanks. okay, so let me ask you one more q, can i go with the telophase synchronised cells for a particular gene expression on that particular phase? i mean can i use a PCR for a particular gene which most probably, i consider, will express during that phase?, without going for isolation!
@veronica martines marignac :) thank you! What if i need to go for protein expression at that stage?
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