We have synthesized a few copper(II) complexes which are soluble in only hot water. We would like to explore their radical scavenging trends. I have gone through a few research papers in which DPPH was dissolved in methanol/ethanol but not in water. Can we use mixture of water and methanol to dissolve the test sample ?
thank you for your response. . this is first time for me to exploring the DPPH assay... if possible kindly guide me to get the clear procedure.
http://www.sciencedirect.com/science/article/pii/S2095177915000131 pls see this link. There are also many other articles.
DPPH is insoluble in Water. I have done sequential extract with pet ether, ethyl acetate and methanol. Now getting high antioxidant activity in ethyl acetate extract than methanol.
When I dissolve DPPH in acidified methanol(15% 1N HCl) it is showing yellow color.
I agree with Arun. You can do DPPH in water as long as you keep a control of just DPPH in water and also use a positive control which can be BHT in water and another one with ethanol. Here is the procedure we used and you can go the original reference from there:
https://www.researchgate.net/publication/264991641_Bimodal-hybrid_heterocyclic_amine_targeting_oxidative_pathways_and_copper_mis-regulation_in_Alzheimer%27s_disease
Hope it works! Good Luck.
Article Bimodal-hybrid heterocyclic amine targeting oxidative pathwa...
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