Can we detect the function of Gram negative bacterial surface protein based on enzyme assay if so what methods can we adopt
There are few enzymes on the outer surface of Gram-negative bacteria, so you could probably make a short list of ones to test.
I have performed 3-point bending on steel beam with 20*20mm square hollow section. I had obtained the Force-displacement graph for the experiment. I am trying to simulate this experiment in Abaqus...
01 May 2024 1,490 1 View
In the course of our experiments, we noted decrease in the pH of R2A agar plates from 7 to 6 subsequent to exposure to gamma irradiation within the range of 15-25 kGy. What could be the reason for...
06 February 2024 7,361 0 View
I would like to seek recommendations from the ResearchGate community regarding the best tool for creating a comprehensive House of Quality (HoQ) diagram. As the HoQ diagram is a crucial component...
15 November 2023 8,993 1 View
Some further explanations are required to describe, how the metal oxide nanoparticles are formed (i.e. where is the oxygen coming from?).
31 October 2023 7,817 2 View
What is scope for research in elearning?
16 September 2023 6,400 4 View
Please respond with reference
03 July 2023 3,057 5 View
Very thin metallic film (few nm) is generally transparent for longer wavelength. Is it true? What is the relation between skin depth, transmittance and thickness of metal thin film ?
11 April 2023 9,320 1 View
I work on Prussian blue dye which has cyanide ligand and coordinated water in it. I want to replace the coordinated water with some other strong ligand
09 April 2023 5,759 0 View
The error message "A fatal error occurred: Failed to connect to ESP32: No serial data received" typically indicates that the computer running the Arduino IDE is not able to communicate with the...
26 March 2023 2,041 0 View
Hi, Need suggestion on the SEM testing. Is measurement model testing is must while wring paper in SEM testing ? or we can directly move to SEM? Regards, Uday Bhale
25 March 2023 4,057 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View