Is there a way to improve the stability of the calcium alginate beads in alkaline pH?

I am working on heavy metal bioremediation using bacterial isolates. I have immobilized bacteria in 2% sodium alginate then cross-linked in 0.2M calcium chloride for 30 min. I have successfully...

04 January 2021 9,094 4 View

How to Strip off HSQ( Fox 16) after etching?

We are finding a hard time to strip of residual HSQ ( Fox 16) after using it as a mask in etching? We are coating 600 nm thick HSQ and writing with 3000 microC/cm^2 dose at 1 nA current. We are...

08 December 2020 5,452 2 View

How to measure the thickness of aluminium oxide made of aluminium coated nonmetal sample?

Aluminum coating has been done on nonmetal substrate. After coating the sample has brought in normal atmosphere ambient. In this condition oxide layer may have been formed. How to measure the...

11 July 2020 4,722 2 View

What is the reason for the low interaction of students in e-learning?

Through our dealings with students, we find a low rate of their interaction with electronic classes, and we note a decrease in the e-learning culture of some teachers. It is also difficult to...

30 April 2020 9,876 18 View

Formation of aggregates while synthesizing DOTAP-based liposomes?

Hello, I am writing this question to this online community in search of any help. I have been trying to synthesize DOTAP ( cationic ) and DOPE ( neutral) based liposomes using (20% of DOTAP in...

17 May 2019 6,233 2 View

What kind of tunneling losses can occur in Multijunction solar cell?

I am investigating different types of losses in multi junction solar cell. I came to know that, tunneling junction is a important factor for designing multi junction solar cell. What kind of...

13 April 2018 591 3 View

I would like to know about the reason for why Helium do not have triple point?

20 October 2017 4,019 2 View

Forest biomass/carbon stock calculation tool for Tier 1 IPCC ?

04 April 2017 6,542 3 View

Which technique from Elecrochemical analyser is used for electrodeposition??

I have electrochemical analyzer of CH instrument 608D.Can Electrodeposition of metal oxides on FTO or Cu are possible by using it ? And which technique from Elecrochemical analyses is used?

02 April 2017 4,691 3 View

How to separate copper sulfate from water?

14 September 2016 568 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View