Trouble shooting with plasmid visualization on agarose gel from E.coli ??

Dear Friends, I am trouble shooting since a long for isolation of my cloned construct in the strain E.oli DH-5 alpha. I have tried Qiagen kit but yield is very low to...

10 November 2019 8,349 2 View

Solution to overcome the diffuse band from SDS-PAGE protein gel and can it be used for western blot?

I have run my Crude extract protein from rice shoot sample and as per my observation I feel that my protein is getting diffused as lower part of my running gel. The gel solutions are freshly...

06 July 2018 3,427 3 View

How to prepare rice leaf sample for confocal image without affecting its cytosolic and organal pH?

Dear Friends, I want to analyse my rice shoot and root tissue for confocal image analysis to look its pH changes through the ratio of emission wavelength intensity of...

02 March 2018 8,219 0 View

Does 10 microlitre reaction of PCR is principally right for qPCR ANALYSIS?

08 September 2016 9,910 3 View

How to purify again to the isolated RNA?

08 September 2016 1,603 5 View

Can any tell me what would be the least possible minimum value of Sodium ion and potassium ion in well processed double distilled water?

07 August 2016 7,944 5 View

How to get actual concentration of metal in plant sample?

05 June 2016 7,236 9 View

Getting only single band in plasmid isolation, why?

Hello Friends, Our lab is facing the problem of visualization of only single band of plasmid in gel instead of multiple bands. While the concentration of plasmid is much enough (around in micro...

09 October 2015 3,474 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View