Amber Gupta

9 Questions 6 Answers 0 Followers

Questions related from Amber Gupta

Trouble shooting with plasmid visualization on agarose gel from E.coli ??

Dear Friends, I am trouble shooting since a long for isolation of my cloned construct in the strain E.oli DH-5 alpha. I have tried Qiagen kit but yield is very low to...

11 November 2019 8,422 2 View

Can we commasive stain the TGX stain free gel after run?

We want to show our protein as loading control and we want to use TGX stain free gel of Biorad. Has anyone experienced with it that it is compatible with Commasive stain after run the gel...

01 January 2019 3,218 0 View

Solution to overcome the diffuse band from SDS-PAGE protein gel and can it be used for western blot?

I have run my Crude extract protein from rice shoot sample and as per my observation I feel that my protein is getting diffused as lower part of my running gel. The gel solutions are freshly...

07 July 2018 3,494 3 View

How to prepare rice leaf sample for confocal image without affecting its cytosolic and organal pH?

Dear Friends, I want to analyse my rice shoot and root tissue for confocal image analysis to look its pH changes through the ratio of emission wavelength intensity of...

03 March 2018 8,270 0 View

How to purify again to the isolated RNA?

Hello Everyone, I have another doubt regarding my RNA sample which 260/280 is quite fine which shows 1.7 to 1.9 value and it shows good band omn agarose gel but bit layer of smear which might be...

09 September 2016 1,694 5 View

Does 10 microlitre reaction of PCR is principally right for qPCR ANALYSIS?

Hello everyone I would like to get suggestion from you people regarding the reaction volume of PCR for my q PCR analysis. I have already cheked my cDNA by actin/18s primer in 10 microlitre...

09 September 2016 9,979 3 View

Can any tell me what would be the least possible minimum value of Sodium ion and potassium ion in well processed double distilled water?

We are analyzing the metal at PPB level through Analytik Zena AAS ZEEnit 700 instrument Recently we have observed the absorbance value of sodium and potassium ion, 0.826 and 0.752 respectively...

08 August 2016 8,000 5 View

How to get actual concentration of metal in plant sample?

Dear friends, Today I have got Atomic absorption data of my plant sample in which I have got one sample showing 497.1 microgram/L means in PPB and the Blank reading is 16 PPB Now I would like to...

06 June 2016 7,315 9 View

Getting only single band in plasmid isolation, why?

Hello Friends, Our lab is facing the problem of visualization of only single band of plasmid in gel instead of multiple bands. While the concentration of plasmid is much enough (around in micro...

10 October 2015 3,547 2 View