Can we apply the second derivative of the bacterial growth curve for strains comparison?

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What are the frameworks or methodologies to examine written academic ELF?

What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.

23 July 2024 4,800 1 View

How to analyse response rates of a questionnaire?

I have developed a questionnaire. I want to try and analyse question drop-off. There is a clear negative correlation between question number and percentage of respondents who answered that...

07 July 2024 9,435 1 View

Can anybody recognize the virus on potato plants?

Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?

19 June 2024 6,739 3 View

Is the preference for more established researchers by reputable academic journals leading to infrequent publishing of Bachelor's degree theses?

I think, for a long time, there has been a tendency for journals to favour work from more established researchers or institutions, thus potentially overlooking quality undergraduate research. This...

16 June 2024 6,801 3 View

How do you generate recursive proofs over time series data?

What are the recommended recursive algorithms to generate proofs of time variant and time invariant properties from a time series data. Approaches like recursive statistical tests, symbolic time...

08 June 2024 4,449 0 View

What is the ratio between the density of the bacterial population during anaerobic and aerobic growth of one species?

What is the relationship between the maximal density of a bacterial population during anaerobic and aerobic growth for a specific species - a facultative anaerobe?

01 June 2024 4,950 4 View

Why do Spanish slugs (Arion vulgaris) prefer to feed on the most poisonous or spicy plants?

Spanish slugs choose to feed on some of the most poisonous and spicy plants. Do they become toxic to potential predators due to this? Are there any publications available on this topic?

28 May 2024 3,929 0 View

How build Forest plot in STATA?

(STATA) Hello everybody. I would like to build a Forest Plot graph that includes all this information from the table. I am unable to build the commands to include this information in the chart....

11 May 2024 6,944 6 View

Does air plasma become optically thick at presssures of about hundred bar?

Is there any experimental or theoretical or theoretical knowledge about the optical depth (may be, for different parts of spectrum) of air plasma at pressures about 100 atmospheres?

19 April 2024 5,539 0 View

Is there any known group of Gymnosporangium sabinae that uses Prynus as the only host plant, both for the telial and the aecial stages?

Gymnosporangium sabinae is a rust fungus known as pear rust. It is a heteroecious plant pathogen with Juniperus sabina (savin juniper) as the main primary (telial) host and Pyrus communis (common...

02 April 2024 1,541 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View