Normally, after extracting a plant with solvents such as ethanol, methanol, and water, the solvent is removed, and the extracts are pulverized. A stock solution is then prepared from the powdered extracts and used in bioactivity studies. Is using the extraction liquid directly in bioactivity studies right without performing this entire procedure? If it can be used directly, how can I calculate how much extract is in this liquid?
To calculate the concentration of extract in the liquid, you would typically perform a quantitative analysis to determine the concentration of the bioactive compound(s) of interest. This can be done using calibration curves prepared from standard solutions of the compound(s) at known concentrations. Once the concentration is determined, you can use this information to dilute the extract to the desired concentration for use in bioactivity studies.
Yes, it is possible to use the extraction liquid directly in bioactivity studies without performing the entire procedure of removing the solvent and pulverizing the extracts. This approach is known as a "stock solution" or "extract solution." The stock solution is prepared by dissolving the extracted material in a solvent, usually water or a buffer solution, to a specific concentration.
To calculate the concentration of the extract in the stock solution, you can use the formula:
C1 V1 = C2 V2
where C1 is the stock concentration, V1 is the volume of the stock solution, C2 is the desired concentration, and V2 is the volume of the stock solution needed.
Abdelhak Maghchiche I think this method is useful for preparing a working solution from the stock solution ( known concentration). However the question is about the concentration of stock solution (C1).
Hello
Yes, liquid extract can be used directly in bioactivity studies in micromiliter quantities.
You just need to follow the written protocols in advance.
Habiba Boukerche If you know of any protocol regarding this issue, can you send it to me?
Ankit Negi Is it correct to calculate how much extractive is in the extraction liquid with the following method?
For example, weigh 1 gram of a plant leaf dried at room temperature and extract it in 100 ml of ethanol. Let the moisture value of 1 gram of plant be 8.50%. In this case, the full dry weight of our plant is 0.915 g. At the end of extraction, the complete dry weight of the plant leaf is 0.892 g. In this case, when we subtract the full dry leaf weight (0,892 g) at the end of extraction from the initial full dry weight (0.915 g), the result we find (0,023 g) will give us the amount of extract in 100 ml. Is this method correct to find out how much extract is in 100 ml?
The concentration of the stock solution (C1) is crucial when preparing working solutions from stock solutions of known concentrations. In the context of bioactivity studies or any scientific experiment that involves dilutions, understanding the initial concentration of the stock solution is fundamental for accurate dilution calculations. This information allows researchers to determine the volume of the stock solution needed to achieve a desired concentration in the final solution.
By utilizing the formula C1V1 = C2V2, where C1 represents the concentration of the stock solution, V1 is the volume of the stock solution, C2 is the desired concentration, and V2 is the volume of the final solution required, scientists can precisely calculate the necessary volumes to prepare solutions of specific concentrations for their experiments. This method ensures the reproducibility and accuracy of experimental results by controlling the concentration of the solutions used in bioactivity studies or other scientific investigations.
- Badges
- Science topic
- Similar topics
- Agricultural Science
- Agronomy
- Crop Science