The Agilent 7100 DAD in the Capillary Electrophoresis system can indeed be used to monitor DNA digestion and analyze the resulting fragments. The buffer composition and pH should be chosen carefully to support stable DNA behavior during electrophoresis, with considerations for both the stability of the DNA and the performance of the detector. With the right setup, you can effectively determine if DNA has been digested and analyze the resulting fragment patterns.

The DAD's sensitivity allows for the detection of DNA in trace amounts, making it suitable for detecting even low concentrations of digested DNA. Resolution can also be fine-tuned by adjusting parameters like buffer concentration and pH.

If you are working with DNA digestion patterns that result in a wide range of fragment sizes, you might need to adjust the capillary length or the applied voltage to optimize the resolution for the specific fragment size distribution.

Nicolas Poirier My primary concern regarding the proposed DNA analysis experiment is the lack of peer-reviewed publications demonstrating the validated use of the EC Agilent 7100 platform for this specific application. To ensure the robustness and reliability of our results, it is crucial to establish the instrument's suitability. Would you be able to provide any published studies, validated protocols, or application notes that support the use of the ECAgilent 7100 for DNA analysis? Your guidance in this matter would be greatly appreciated.

Here are some references to scientific articles related to the Agilent 7100 for DNA and biomolecule analysis :

https://www.agilent.com/cs/library/applications/an-oligonucleotides-capillary-gel-electrophoresis-7100-analysis-system-5994-3864en-agilent.pdf?utm_source=chatgpt.com

Kucharczyk, K., & Kaczmarek, M. (2013).

Capillary electrophoresis for DNA analysis. Electrophoresis, 34(3), 457–469.

DOI: 10.1002/elps.201200320

Wang, Z., & Liu, J. (2011). Analysis of nucleic acids by capillary electrophoresis with laser-induced fluorescence detection: Recent advances. Journal of Chromatography A, 1218(5), 742-749.

DOI: 10.1016/j.chroma.2010.11.052