Any suggestions?
Without the filter you will see the UV tubes. You can get a less bright image with SYBR and the EthBr filter.
I have done DGGE gel analysis for three different genes from ten rhizosphere plants. I have got the data on band intensity and SW index and I would like to do Principal Component analysis (PCA)...
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I calculated diversity index of DGGE profile by using bionumerics software. the index range for Shannon wiener is the following for five different rhizopshere SW index ...
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For determining the DNA DNA hybridization between two type strain species Can SYBR qPCR master be used or only SYBR GREEN nucleic acid stain can be used?
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How do you remove humic acids from soil DNA?
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I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
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How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
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Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
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kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View