kind suggestion to you sir ,please stop working on Lipase project it is of time waste ,better choose another project which can be concluded.

Telling you with personal experience

You can use glycerol tristerate as true lipase substrate and reference of Qazi et al for method .But I want to give good suggestion to S Reddy, that if you are able to click in science at right time you are achiever and get success, other wise not criticize it develop patience and focus on target. 

Mr chand raina Why are you feeling so bad ,I have worked in reputed organisation with same project for several years we have tried with all atl ast nothing was concluded so I adviced benjamin.

http://www.ncbi.nlm.nih.gov/pubmed/16637669

Thanks to each and every one for your contributions and suggestions. Most especially to Precious, Chand Raina, Sekhar Reddy. Your suggestions and advice are welcomed.

Thanks

For lipase assay I would like to advise to to follow winkler and stuckman 1979 methos as given in these papers.

https://www.researchgate.net/publication/47644372_Synthesis_of_ethyl_ferulate_in_organic_medium_using_Celite-immobilized_lipase

https://www.researchgate.net/publication/224900014_Catalytic_Potential_of_a_Nitrocellulose_Membrane-_Immobilized_Lipase_in_Aqueous_and_Organic_Media?ev=prf_pub

https://www.researchgate.net/publication/284078828_Cellulose_binding_domain_assisted_immobilization_of_lipase_GSlip-CBD_onto_cellulosic_nanogel_Characterization_and_application_in_organic_medium?ev=prf_pub

Could I get some advice on Lipase Assay? - ResearchGate. Available from: https://www.researchgate.net/post/Could_I_get_some_advice_on_Lipase_Assay#58a2c1b7dc332d157d7afa63 [accessed Feb 14, 2017].

Article Synthesis of ethyl ferulate in organic medium using Celite-i...

Article Catalytic Potential of a Nitrocellulose Membrane- Immobilize...

Article Cellulose binding domain assisted immobilization of lipase (...