MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

How can I estimate and plot Logarithmic Error Bars (in Excel, preferably)?

Dear Dr.colleagues, I am currently plotting the dynamics of an infection in Excel. For each sampling day, I plotted the mean infection load of the population, using the standard error for the...

07 January 2021 2,069 3 View

Is GPD 2021 an official and real event?

I was invited to this conference organized by the Hazel pharmaceutical group in Prague 2021. I want to know if the event is real and if any other collage in the pharmacology/ drug discovery area...

25 December 2020 4,407 3 View

What "planned comparisons of LS means" in Statistica is doing?

Dear colleagues, I am tryng to understand what the option "planned comparisons of LS means" in Statistica is doing? Let's say I have a factorial ANOVA with two categorical explanatory variables:...

06 December 2020 9,272 4 View

What's the best multiplex RT-PCR detection system to buy?

Is someone working with the CFX96Touch Real-TimePCR System (Biorad)? How is your experience with this system? It is our first option for multiplexing qPCR systems because it is in our budget range...

27 September 2020 5,558 4 View

How can I include mean hardness in a formula with time and volume to calculate disintegration?

Dear math smarts colleagues, So, after disintegrating a hard material I obtain the total energy employed by the breaking device (laser), the minutes or seconds it took, and I know in advance the...

30 August 2020 1,299 3 View

Anyone interested in the field of Human Reproductive Biology looking forward to collaborate in clinical research?

Fertility Center - IECH, Monterrey, Mx.

07 July 2020 3,006 5 View

Why do washing machines wash so long?

Programs on my new washing machines last 2 hours or notably more (at low temperatures). There is an option "timecare", but it does not change duration notably. I asked in the shop; they say that...

03 May 2020 5,044 39 View

What is the difference between unstable crack growth and ("stable") rapid crack propagation (RCP)?

I have read in some articles about unstable crack growth which is sometimes referred to as RCP, however it seems that there is a clear difference between unstable crack growth and RCP. According...

26 April 2020 513 3 View

White coat on ZnSe surface, what is it?

I'm working with a ZnSe window and, during my process, I observed a white coat on the window's surface. It's uniform with some clear spots that look like a drop. It could have been methanol or...

11 February 2020 541 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View