Can someone suggest some literature on efflux mechanisms of excess intracellular calcium in cardiomyocytes?

More Krishna Chander Sridhar's questions See All

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How does a Man-in-the-Middle (MitM) attack work in the context of Transport Layer Security (TLS), and what specific mechanisms can be employed ?

Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...

19 June 2024 6,595 3 View

Why is colony PCR not working ?

I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...

07 June 2024 203 2 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

If my standard quercetin equation is Y = 0.0057x + 0.0635 then how can I know the value of ethanolic extract of 10 mg/ml if absorbance value is 0.39?

I wanted to know the value of total flavonoid content in mgQE/gm. Total volume I kept in well plate is 200 µl in which extract was 20 µl. Anyone please help me with this problem

10 May 2024 5,658 4 View

What will be the value of potassium content in mg/100g if absorbance value is 45 ppm in flame photometer?

Amount of fruit powder used 0.15g; final volume of the extract was 100 ml and I have done my experiment in flame photometer. I did not know how to convert 45ppm in mg/100g. Please help me?

10 May 2024 3,173 0 View

Difficulty in Phase separation ?

Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...

17 April 2024 8,522 3 View

Suggestions required for use of Ion exchange resin?

For purification of carbohydrases (carbohydrate degrading enzymes) from bacterial cell free supernatant (CFS), which Ion exchange resin can be used? Are there prepacked columns available for the...

14 April 2024 3,699 0 View

Question: Herbicide formulations ?

Which is probably a better and efficient formulation for the application of Pre-emergent herbicide? A) Dry formulation B) Liquid formulation?

11 April 2024 5,157 1 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Are diatom inclusions found in shellfish shells?

Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...

12 July 2024 159 2 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Initial and final setting times of a calcium phosphate cement?

I'm measuring the setting time of a calcium phosphate cement using an apparatus that records how much resistance a needle (connected to a load cell) encounters over time. This process provides...

24 June 2024 7,534 5 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

After plating mouse primary neonatal cardiomyocyte we do not observe beating after a few days, any suggestions as to how we can trouble shoot?

We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...

20 June 2024 393 0 View

Markers to distinguish individual cardiomyocytes?

I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...

20 June 2024 3,225 2 View