I want to purchase HIF-1a (CA5) vector for constitutive expression of HIF-1a. Could anyone suggest me where to purchase it from.
If you can find this vector in a published study using a pubmed search, you can email a request to the corresponding author.
Otherwise, non-profit plasmid depositories such as Addgene may have it.
Hi Arjan
Thank you for the answer. But the plasmid you suggested seems to retain the oxygen-dependent degradation domain (ODD). Do you think the mutation P402A, P564A alone is sufficient for oxygen resistance?
What do you think of
https://www.addgene.org/52216/
Thank You!
Great Arjan! Of course I need a functional HIF-1a to study its downstream signaling.
Thnx for your suggestion!
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