I want to purchase HIF-1a (CA5) vector for constitutive expression of HIF-1a. Could anyone suggest me where to purchase it from.
If you can find this vector in a published study using a pubmed search, you can email a request to the corresponding author.
Otherwise, non-profit plasmid depositories such as Addgene may have it.
Hi Arjan
Thank you for the answer. But the plasmid you suggested seems to retain the oxygen-dependent degradation domain (ODD). Do you think the mutation P402A, P564A alone is sufficient for oxygen resistance?
What do you think of
https://www.addgene.org/52216/
Thank You!
Great Arjan! Of course I need a functional HIF-1a to study its downstream signaling.
Thnx for your suggestion!
I am trying to knockdown my gene of interest in P4 mice using liposomes. I injected the control or siRNA intraperitoneally (in 30 ul volume) for two consecutive days. Retinal vessels were analyzed...
04 May 2014 1,981 2 View
I am trying to do western blotting of tumor samples for HIF-1a. The antibody is from neomarkers and I used a dilution of 1:1000. The protein samples from the tumor were isolated in RIPA buffer. I...
02 March 2014 2,459 13 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...
01 August 2024 6,737 1 View