I have synthesized Phthalated chitosan, now want to calculate its degree of substitution, is their any method available for calculating degree of substitution by UV spectrphotometry or Tirimetry????
Please help me out.....
This is a tricky question. UV measurements can be quite sensity to low level contaminants. When a yellow or brown colored material is formed during the synthesis, which often happens, then a quantification by UV measurements is not possible due to an high background signal.
P.S. The DS of cellulose ethers is usually measured by the Zeisel method. Ether linkages are converted with HI to substituent-jodides and these are quantified by GC,
Possibly, this can be quantitatively done by 1H NMR, and can also be selectively quantified between N-substitution and O-substitution of chitosan. First, the structure of the phthalated chitosan needs to be confirmed through 1H, 13C, and 2D NMR.
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