Gi often get nice pellets forming but they sometimes don't seem to run when I tilt the plate, should I count them as antibody positive.for example streaming ofpellets has occurred up until the 1:160 dilution but the pellet in the 1:320 looks real but doesn't stream
Also both in the agglutinating and pelletet wells the cells seem to lyse giving an odd smear in the wells after 5 or so minutes following settling other RBC control
Amy ideas?
Hi Mark,
If I was reading a plate I would only count up until the well that runs (the "teardrop") and anything after that doesn't stream I wouldn't count.
You should read your plate immediately (if you don't have time to calculate the HI then just draw a line with pen so you can come back and calculate it later.) If you leave it even for 5 minutes or less they all begin to stream, so you should read your controls at the same time so that the teardrops are all forming at the same time.
Don't set your plate back down after you have tipped it, read it ahd mark the plate immediately.
Hope this helps!
Joanna, I only see the lysis in the serum samples not when I titrate the virus and not in the RBC only wells on the hi plate.
Do you know what it is about the serum that causes lysis - just curious
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