Can someone help me why air bubbles form during streaking on agar plates?

More Vaibhav Sunil Tambat's questions See All

Collaboration with researchers from different countries asking for the morphometric data based on herbarium samples?

Dear Colleagues: Good day and greetings from CSMCRI, India! I am thankful to all those who have responded to my previous query posted in the list last year. We are pleased to inform that our work...

19 June 2024 2,468 0 View

What are the main causes of climate change due to heat waves ?

* I want to know, what are the main factors responsible for changing climate ? * What are useful remidies to overcome or manage global warming ?

30 May 2024 10,118 2 View

Current Trends in IoT Automation?

I am currently delving into the latest trends in IoT automation and am keen to gather insights on the most promising developments in this area. Could you share your experiences or recent findings...

24 April 2024 7,509 0 View

Impact of AI Tools on Academic Research?

As AI technology, such as ChatGPT, continues to advance, I'm curious about its impact on academic research. How do researchers view the integration of AI tools in their work? Are these tools seen...

23 April 2024 7,832 4 View

Soil Physics Problem?

A sprinkler adds 15 cm of a bromide solution (i.e.,15 cm3 of water for each 1 cm of area) at a rate of 2 cm h-1. The volumetric water content (Theta) of the soil is 0.30 cm3 cm-3. Assume...

27 January 2024 7,107 1 View

If anyone can solve this problem in soil physics, please let me know?

Imagine you have a field with excessive salt buildup. Assume the volumetric water content is 0.35 cm3 cm-3 and the root zone is 120 cm deep. If the soil in the field is a clay loam soil with Ks=...

20 January 2024 6,449 4 View

Why my plasmid construct is not expressing after cloning?

I have been trying to express my construct for the last couple of weeks but after several attempts, it's still failing although similar parts (five inserts for golden gate assembly) have been used...

12 December 2023 4,729 8 View

How to do proper cloning calculations for my plasmid construction pcr reaction?

Hi, I have recently started my cloning experments and don't have much experience in doing ligation reactions. I have five insert parts with each having 10ng/ul concentration and i need 200ng of...

03 December 2023 1,786 4 View

How to resolve issue in computation of nodal forces in LS-Dyna?

Greetings to All, I am currently conducting a steady-state frequency response analysis of a human body finite element model using LS-Dyna. I have applied an acceleration load and obtained the...

03 November 2023 5,428 1 View

How to evaluate or check the animation outputs (Motion) of Frequency domain response in LS-Dyna?

Dear All, I am performing the frequency response analysis of the human body FE model using steady-state dynamics. I have given base acceleration in terms of amplitude and phase angle. The output...

08 October 2023 9,735 0 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Masonry Interaction Module ABAQUS?

I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...

16 July 2024 5,047 0 View

How indoor air pollution is sampled using TSI quest EVM air monitoring instrument?

please suggest the research paper related to the instrument is used in it.

16 July 2024 8,453 1 View

Ivan Salazar Alcaraz

Do not shake the medium vigorously before pouring it into the plates.

Be careful when pouring into the plates, do not pour from a great height.

Vaibhav Sunil Tambat

Ivan Salazar Alcaraz but during pouring, there is no bubble formation, it is similar to normal media plates this thing only happens after streaking of microalgae culture. so there any particular reason behind this?