I am currently working on purifying DPP4 inhibitor from natural resource. But facing lot of problem using plasma as a source of enzyme.
Can someone please help in getting purified enzyme so that i can proceed further in purification. Also i would like to learn about cloning and expression of DPP4. If someone can share protocol it would be a great help for me in carrying out my PhD work.
I usually find google scholar to be helpful.
http://www.ajol.info/index.php/tjpr/article/view/107629
Also sometimes if you find a reagent company that sells it you can save yourself a lot of time and effort that cloning and protein purification requires and keep your focus on what will help you complete your thesis.
https://www.rndsystems.com/products/recombinant-human-dppiv-cd26-protein-cf_1180-se
Hi there,
DPP4 is a membrane protein which means it is making things more complicated about expression/purification. Some have purified from transfected insect cells:
http://www.sciencedirect.com/science/article/pii/S1046592802000438
Some have tried to purify the functional exodomain from yeast:
http://www.ajol.info/index.php/tjpr/article/viewFile/107629/97481
Thankyou Dominique for an article. Ya its difficult to purify thats why trying to seek help from experts.
Thankyou joe. Actually its matter of fund. I just wanted to give a try otherwise i was using plasma as source.
please you can see this link may be help you
http://www.uniprot.org/uniprot/P27487
http://www.proteinatlas.org/ENSG00000197635-DPP4/tissue
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