Interpretation of this compound and all the chemical shifts
See attached file I made using ChemDraw Pro version 15.1.0.144.
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
I am deciding on search terms for a scoping review. I am planning searching 3 multidisciplinary databases and currently getting about 5000 hits from my search terms in the first database. What do...
19 June 2024 7,417 0 View
Hello, I'm relatively new to qPCR and have been utilizing a manufacturer's analysis software for my experiments, including one-way ANOVA for statistical analysis. However, I've encountered a...
14 May 2024 3,307 3 View
Chi2 should be used for groups which count at least 5. What if one of the four groups compared is smaller? Additional question: any post-hoc test for comparison between groups? Thank you!
13 May 2024 1,425 2 View
The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...
12 March 2024 3,926 0 View
cell culture: HEK293F medium: BalanCD, without antibiotics
30 March 2023 8,850 1 View
In my case: LiBH4 - LiBr in different molar ratio. I have the observed patterns (measured by XRD technique) for this mixture of compounds but I don't have .cif file or any reference to compare...
03 March 2023 2,640 4 View
Hi, I recently started doing a lot of IHC and I’ve been staining mouse coronal section (not in parafin) with 3D6 antibody to visualize plaques. My problem is that the background is too dark and...
02 December 2022 6,768 1 View
Dear all, Have you ever used kisspeptin antibody antiserum AC566 rabbit anti mouse? I wanto to try immunfluorescence on the mouse brain tissue but I don't have much expereince with this method....
21 October 2020 5,218 0 View
I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...
30 September 2020 2,731 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View