Hello,
I am planning to perform a death assay with M93 cells and looking for a good protocol, and in addition, how to plate cells for a death assay?
IC 50 protocol
My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.
24 February 2021 393 14 View
I am working on a project monitoring crop field health. For this purpose, I am preparing my dataset using drone video. I have converted it into keyframes. Now I want to pre-process these aerial...
25 December 2020 7,529 5 View
I want to know about the dangling bond? by which method we can find it, does its need some special type of characterization just like XRD etc... or method i want to find dangling bond effect in...
26 October 2020 1,938 6 View
Hi, can someone explain which algorithm should I use to convert DN(Digital Number) values to radiance, because there are many different algorithm that I found, there are 3 for now (based on...
29 August 2020 1,479 2 View
During this pandemic caused by COVID_19 there has been a trend in the academics also due to the modernization by attending online Webinars and even some international conferences are being...
09 August 2020 342 17 View
Can you please erase the doubt from my mind regarding 'Pay and Publish' versus 'Free Publication,' because in both the cases money is being demanded either from the author or the reader. In the...
02 August 2020 1,536 8 View
What are the best tactics, suggestions and recommendations that one should adopt while converting a Ph.D. thesis into a Book and who can be the genuine or reputed publishers for it?
02 August 2020 5,260 14 View
I'm currently doing a study on potential hydrogen damage in superalloys. I'm wondering if carbide dissolution takes place in superalloy in the same manner as steel does with the potential...
12 July 2020 1,420 1 View
Hi everyone I need help in preparation samples for RAMAN spectroscopy, i am working in thin film field, spin coated on ITO or Si wafer. Can any one suggest RAMAN sample preparation method ?...
25 May 2020 7,050 9 View
Dear All, I got 2 weeks old paraffin blocks of tumor masses. I want to extract total RNA from them. The available reagents in my lab are: 1) Cell dissociation buffer (GIBCO#13151-014) 2) TRI...
30 March 2020 5,067 5 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View