When i prepared primary emulsion (w/o) white layer is precipitated. It may be either PCL or drug. I did not use surfactant for making primary emulsion. My drug is highly water soluble.
You should describe more in detail and with more appropriate "terms" in order to have a reasonable answer.
dear claudio.... PCl is hydrophobic in nature and my drug is hydrophilic in nature. so in order to prepare nanoparticles, by w/o/w multiple emulsion, drug was dissolved in 1ml of water and emulsified it with 10 ml of dichloromethane using probe sonicator. But I could not get proper emulsion. A thin polymeric film seprated in emulsion. wbat could be the solution for this.
Dear Bhavin, I prepared nanocapsules (w/o/w), with a hydrolifilic drug + iron oxide nanoparticles in a sodium citrate solution + PCL(or PLGA) in dichloromethane + a aqueous solution of Pluronic F-68, this first mixture was sonicated (w/o), then the first emulsion was sonicated with a PVA aqueous solution (w/o/w).
Then, I have in the w1 (drug + iron oxide + sodium citrate aqueous solution + Pluronic aqueous solution)
in oil phase (PCL + dichloromethane)
in w2 (PVA aqueous phase)
This formulation is OK for PCL and for PLGA!
Dear Siram,
Thank You for your suggestion.
What could be the justification for using ethyl acetate?
In my research, ethyl acetate is excelent for PLGA, but with PCL, I could never achieved a good solubilization in the range of 25 mg/mL to 200 mg/mL.
Have you had sucess with DCM and PCL, Siram Karthik? Which concentrations?
i tried three solvent for pcl till now. DCM, ACN and , DCM+ACN(1:1). In all cases i am facing same problem. Now, i am going to use ethyl acetate and also method suggested by marcela using poloxamer. thanks to both of you.
For water soluble drug try for DCM as Intermixing or penetration of ACN will be more. So try for DCM only.
@marcela Yes, I have tried DCM and PCL. But the particle size is not good. But PCL and ethyl acetate produced particles with a size less than 100nm. Hence I have worked only with PCL and ethyl acetate.
- Badges
- Science topic
- Similar topics
- Gels