I am using a QuickTiter Retrovirus Quantitation Kit and the protocol says to "read the microplate using a 480/520 nm filter set", but I am unsure as to what this means. Is this suggesting that there should be two excitation wavelengths, or that one wavelength is for background? I am currently using a SpecrtaMax L luminescence microplate reader with Softmax Pro software and I cannot determine the correct settings to use.
Tarek ABD EL AZIZ
I am sorry the answer is too late.
I used another software to read my micro plate according to this parameters (excitation wavelength at 485 nm, emission at 520 nm, and G-Factor at 0.98). I hope this measurement parameters will help you.
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