Joshua Lee

2 Questions 2 Answers 0 Followers

Questions related from Joshua Lee

Lipofection or electroporation for a 12 kbp plasmid transfection in a fibroblast cell line that displays impaired membrane resealing?

I'm trying to transfect a plasmid construct (about 12 kbp) into a fibroblast cell line that displays impaired membrane resealing. Considering this impaired resealing ability, I want to chose a...

04 April 2015 2,761 7 View

Can someone explain the correct settings for a fluorescence plate reader for use in retroviral quantitation?

I am using a QuickTiter Retrovirus Quantitation Kit and the protocol says to "read the microplate using a 480/520 nm filter set", but I am unsure as to what this means. Is this suggesting that...

05 May 2014 4,705 1 View