We need to collect a sample of bone marrow from mice post mortem. We haven't done this before and wonder if people who have experience of doing it have any advice on exactly how it should be done and any precautions we should take.
Depends on what your'e looking for. For flow cytometry, bone marrow transplant or in vitro analysis, I would remove the femur, trim off fat/muscle, cut off the ends while in media and flush with a syringe. Depending on the next step, you may need to filter the suspension through a 70um filter. You can always save the other femur in 10% Formalin for paraffin embedding for H&E cuts or IHC.
Depends on what your'e looking for. For flow cytometry, bone marrow transplant or in vitro analysis, I would remove the femur, trim off fat/muscle, cut off the ends while in media and flush with a syringe. Depending on the next step, you may need to filter the suspension through a 70um filter. You can always save the other femur in 10% Formalin for paraffin embedding for H&E cuts or IHC.
Thanks for the reply. we are proposing to measure telomere lengths, and were informed that bone marrow is the best material to do this
This might be helpful as well, a link for the journal of visualised experiments (JoVE), which shows the procedure:
http://www.jove.com/search?q=bone%20marrow
Basil
John great that you are doing TL in bone marrow, will you also do TL from white blood cells at the same time? Would be an interesting comparison. If you know papers that did such a comparison already I would be interested. Also note that my personal opinion is that dynamics of TL are usually more interesting than a terminal sample of bone marrow.
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