I have no personal experience, but it seems people generally do ELISAs for this. Here are some providers of kits and antibodies for ELISA:

http://www.sunredbio.com/eproductlist.asp?id=8914

http://www.genetex.com/4-Hydroxynonenal-antibody-GTX40953.html

http://www.raybiotech.com/goat-anti-4-hydroxynonenal.html

http://www.abnova.com/products/products_detail.asp?Catalog_id=PAB13866

Hi Elnaz,

I am not sure if you are still looking for this antibody, but we just launched an antibody for 4-HNE that has been validated for use in ICC/IF, WB, and ELISA. It's species independent, so it should work for you. Here's the info:

4-Hydroxynonenal Antibody [12F7] Catalog No. SMC-511

• http://www.stressmarq.com/products/antibodies/4-hydroxynonenal-antibody-smc-511/

We also offer a free sample program, so you can try a 12ug vial of this antibody and only pay for shipping to your institution. Here's a link to the full info on our free antibody samples:

• http://www.stressmarq.com/free-antibody-samples/

Best of luck!

Leslie