Why a previously protein expressing same gene construct in pET vector (BL21 host) is not showing protein expression?

I am facing problem in protein expression from the gene cloned in pET28 vector (BL21 host) which was previously expressing happily. Now I am trying to induce protein from same construct in same...

05 June 2015 3,231 11 View

How do I identify correct recombination events worked out using RDP 4 software?

I am trying to find out recombination among viral sequences using RDP 4 software. With the aid of this software I identified two about 30 recombination events. Out these 30 recombination events...

02 March 2015 7,633 17 View

Can we get more than one dissociation curve during real time PCR for one sample contaminated with genomic DNA from other sources ?

I just want to know whether the real time PCR will show different denaturation curves for single sample contaminated with different genomic DNA from different organismal sources? Suppose I have...

03 April 2014 8,475 5 View

Why is the 16s rDNA sequence from endosymbiotic bacteria not getting amplified by PCR?

I am trying to amplify via PCR the 16s rDNA sequence from endosymbiotic bacteria of insects. I tried different concentrations and dilutions of the template. Initially, it was showing good...

01 February 2014 8,170 3 View

What is the optimum temperature for pollen germination, pollen viability, and stigma receptivity in cotton flowers?

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09 October 2013 907 0 View

Is there any problem in ligation of sma1 and ecor1 insert with vector cut with same res. ezymes?

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09 October 2013 3,154 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View