Can somebody suggest me some good literature for conducting BRET (Bioluminiscence Resonance Energy Transfer) assays?

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Rob Keller

Dear Puneeta,

A good way to start will be the following reference:

https://www.nature.com/articles/nprot.2006.52

Corresponding to:

Pfleger, K. D., Seeber, R. M., & Eidne, K. A. (2006). Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions. Nature protocols, 1(1), 337.

See for an example: https://journals.sagepub.com/doi/pdf/10.1177/1087057108324032

Good luck!

Carolina Montanez

In our lab we do kinetic BRET and static BRET. For kinetic BRET: Article RGS14 regulates the lifetime of Gα‐GTP signaling but does no...

For static BRET, I recommend this link: Article Bioluminescence Resonance Energy Transfer to Detect Protein-...

Puneeta Bathla

Carolina Montanez , Thanks a lot for providing the useful links ! :)