How can I prevent gene expression before collecting microorganisms by centrifugation?

Hy everybody, I am looking for a way to stop gene expression during harvesting of microalgae to ensure reliable RNAseq in response to temperature. I am affraid working on ice and performing...

25 November 2020 7,282 3 View

How to increase the efficiency of overexpression using lentiviral TET-ON system in primary kidney cancer cells?

I am using a lentiviral TET-ON system for conditional overexpression of my gene of interest in primary kidney cancer cells. I use viral load from transfection of HEK293T cells after 48 and 72...

16 November 2020 2,496 1 View

Has any endophytic microbe been isolated from the bark of coffee plant?

Any bacteria or Fungi from the barks of coffee

10 September 2018 2,993 4 View

Very minimal researches published on online businesses even though online businesses is gaining moment world wide due to ease access of the internet?

Very minimal researches published on online businesses even though online businesses are gaining momentum world wide due to ease access of the internet?

22 May 2018 4,022 3 View

What is the maximum solubility of RNA ?

I am looking for publications (or patents) stating the solubility of RNA (either total RNA, tRNA, whatever the species) in water or in saline solutions. Thanks for helping me !

19 September 2017 9,880 5 View

Can someone provide a protocol for building yeast strains using Tet-Off expression system?

I am trying to express my gene of interest using a Tetracycline inducible vector. Although I need my gene of interest of be off and only be turned on for a short period of time when needed, I only...

07 July 2017 3,994 19 View

How to troubleshoot qPCR primer optimizations?

I am having some issues with primer optimization for qPCR. Typically, I first test various concentrations of primers to figure out which concentrations gives the best melting curves and the lowest...

17 January 2017 9,923 2 View

Xylan from beechwood discontinued by Sigma-Aldrich

28 August 2016 3,993 7 View

Hi! can anyone help me identify this plant from a lake in the Philippines which is constantly being affected by fish kill?

07 January 2016 2,793 3 View

Do plasmids need to be CIP treated for plasmid religation assay?

I am trying an experiment in which I measure the NHEJ efficiency of a mutant yeast strain and compare to wild-type to analyze whether the mutation affects the NHEJ process. I take a low copy...

16 December 2015 2,586 3 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

How does trust influence the development of environmental projects?

Hello Scholars, I am exploring "trust" relevance in the development of socio-technical systems. What is your perception of cognitive values like trust to the energy transition/implementation of...

09 February 2021 8,133 5 View

MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View

Greetings Doctors, If i did a same experiment for a 2 populations and i need to publish this work in a 2 different papers?

If i did a same experiment for a 2 populations and i need to publish this work in a 2 different papers can i use the same word in the writing of these 2 papers or this would considered as...

04 February 2021 4,648 4 View

Can you recommend a good book on analytical chemistry for non-chemists?

Dear colleagues, I am asking for you recommendation: I need a book on analytical chemistry in environmental sciences. As a non-chemist (a biologist) I have some basic chemistry knowledge but not...

25 January 2021 8,486 8 View

I need books or anything else to help me to understand options and others financial derivatives products in english or french?

I am studying at Laval university and one of my course is about options and financial derivatives products , I really have some difficult to understand that course so I am looking for any...

20 January 2021 3,742 3 View

Which statistical test to use to see if there is a significant difference between the vigilance of bird species at different feeders (in SPSS)?

I have gathered data on bird species visiting 4 hanging feeders in different locations and have calculated the proportion of time they have spent displaying vigilant behaviour while at the feeder....

18 January 2021 9,959 7 View