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Thanks a lot. Can you sent to me that which you have optimized in your lab. Will be most grateful.
Walter
I have just received my NGS data files in FASTQ format. Is there any free software that I can use to download and view the files?
01 February 2014 5,037 11 View
I am interested in the esterification of whey proteins and have read in articles that one of the most important steps is the addition of hydrochloric acid equivalent to 50 molar ratio of acidity...
01 February 2014 7,275 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
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11 August 2024 5,138 1 View
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10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
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08 August 2024 9,882 2 View
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07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
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06 August 2024 1,367 3 View
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04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View