We've been dealing with a persistent contamination issue in our E. histolytica cell cultures. We suspect it is a fungal contamination, but identifying the specific type of organism has proven to be challenging. Our goal is to accurately determine which microorganism is responsible in order to apply an effective treatment.
Has anyone experienced this type of contamination? We would greatly appreciate any advice beyond the standard protocols, as we have already implemented common measures such as treatment with amphotericin, disposal of potentially contaminated materials, and strict cleaning and disinfection of the culture area.
It's difficult to tell from the pictures, but in your first (pargest) pic I think I can see budding chains, which makes me suspicious of a Candida infection. Not sure if it will harm your E histolytica, but you could try diflucan (fluconazole)
Hopefully your cell culture seed stock (usually stored frozen) inoculum is not contaminated; might be prudent to check on possible contamination of your cell culture inoculum (there are standard protocols for this).
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