Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Ferret PBMC Fc block?

Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...

18 January 2021 4,504 5 View

Platelet Counting on Flow Cytometry?

Hi, Does anyone have a method for counting platelets on flow cytometry (cytometer=Cytoflex) without the need of using TRUcount beads or reference beads? Is it possible or do I need to purchase...

23 September 2020 3,509 1 View

Can I employ clustering method on abundance-based dataset that has no information about species richness?

Dear friends, we have sampled a taxonomic group in 40 different sites. The data set is abundance-based without any info on species richness. What is the best statistical approach to cluster the...

07 August 2020 1,073 5 View

What is the best statical method for comparison of performance?

Suppose that i want to compare the accuracy of prediction between A and B. The number pf experiment actually is more than 100 experiments. I only put 6 experiments for example. If i use the...

13 May 2020 1,466 3 View

Hai everyone I have one question related to power of study and sample size?

In my study, I used single proportion formula: Sample size, n = Z2 P (1-P) / d2 Where Z = 1.96, d = 10% P = 10%, which is the increment in postprandial metabolic rate after consumption of LCS...

12 May 2020 1,960 3 View

What is the best bibliographic output style for preparing a CV?

I am looking for an output style in Endnote to list publications in one's CV. These would have to be be listed in reverese chronological order. Recommendations and opinions welcome.

23 March 2020 1,228 4 View

ACE Inhibition Assay?

1) I am curious on what solution should I used to dilute the ACE enzyme to respected concentration? is it the same regardless of what solution? or the activity will be differ accordingly to the...

24 February 2020 9,530 2 View

Anyone using stereo trail cameras (camera traps) linked to gather morphometric data from cryptic species?

Looking to connect with any research, papers or groups using remotely triggered stereo cameras to create 3 dimensional pictures of wildlife and gather data on cryptic species. Wanting to set up a...

01 February 2020 940 3 View

How to create colored disease maps?

I have 9 years disease data and I wanted to create maps showing incidence of a disease from different districts at country level on yearly basis. Previously I have been using CorelDraw to draw...

20 November 2019 6,671 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View