Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

How to generate topology file for ligand for Gromacs?

Dear all, I converted a PDB file into Mol2 file using Openbabel (H added) and then fixed the bond order number using this command: perl sort_mol2_bonds.pl jz4.mol2 jz4_fix.mol2 When I try to...

22 December 2020 303 4 View

Can we used PTOTACs to improves the RNA vaccines immunity period ?

Using targeted degradation of non structural proteins of viral capsid can we lengthen the vaccines effectiveness

21 December 2020 7,200 1 View

Can I reuse the NAP-5 colums(GE)?

Hi all, I am using NAP-5 disposable columns from GE for removing DTT from my protein. Can I use the same column again for the purpose of removing DTT. Can you please mention the protocol for...

10 December 2020 3,199 5 View

How to reduce (which reducing agent) a disulfide bridge in a protein and how long does it remain reduced?

I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...

24 November 2020 3,211 5 View

Is it possible to get higher order of diffraction (n>1) in HRTEM?

The SAED pattern, we obtained from HRTEM shows the interplanar spacing (d) that corresponds to a higher 2theta value than the Powder crystal X-ray diffraction pattern for the same organic...

02 November 2020 8,073 3 View

What are the efficient methods available to test the soil fertility?

Want to understand the scientific mechanisms to know type of soil suitable for agricultural purpose.

22 October 2020 4,941 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

08 February 2021 9,070 5 View

Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View

Why does sodium metabisulfite generate turbidity when mixing with sodium hydroxide?

When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...

03 February 2021 8,075 2 View

I want to extract engine oil from a soil sample,please how much amount of anhydrous sodium sulphate am i going to add to 10kg soil?

Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate

01 February 2021 9,509 1 View