It is mentioned in the Procedure of Prieto et al., 1999 that sodium Phosphate should be used. The question is whether it is sodium tri-phosphate or sodium phosphate monobasic.
During the preparation of a ligand molecule in autodock or openbabel Cl atom is not showing in the .pdbqt file whereas it was present in the pdb and sdf file. Due to this Cl atom is not showing in...
13 July 2024 3,486 6 View
This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...
10 July 2024 5,966 2 View
I need some mask for UV photolithography. Can you suggest any software to draw those patterns with proper file format so that directly the file can be inserted and required pattern can be...
22 May 2024 9,449 3 View
I need a cracked version of origin pro and casaXPS for MacBook AIR M1
12 May 2024 7,259 0 View
In order to find the band gap, I plotted a tauc plot of a UV absorption data. The Tauc plot contains two slopes as shown in the figure. Kindly tell me how to obtain the correct optical band gap...
12 May 2024 5,449 3 View
Can we use panel ARDL model with dummy if we have T=20 and N=8, given the condition that DV is I(0) and IV are mix of I(1) and I(0)...
07 May 2024 943 0 View
I plan to do ELISA, GSH, and NO assay from mice hippocampus. However, because of limited tissue, I can't homogenise tissue according to different protocols. By doing some literature, below is the...
15 April 2024 8,423 0 View
I have analyzed groundwater of the middle indo-gangetic plain and detected the Na ion concentration (90 mg/L) and Cl ion (1.7 mg/L). Is it possible?
12 April 2024 3,452 6 View
in optimization process its showing for carbohydrates computational studies. How to Solve Inaccurate quadrature in CalDSu. errors in Gaussian?
05 April 2024 1,389 1 View
We followed the NREL laboratory protocol to analyze reducing sugar after pretreatment of biomass via DNS method. But everytime we found the UV-Abs peak at 370 instead of 540nm. Suggestions are...
15 February 2024 2,605 0 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...
07 July 2024 468 0 View
Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...
07 July 2024 8,626 2 View
I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...
26 June 2024 4,522 0 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
I'm measuring the setting time of a calcium phosphate cement using an apparatus that records how much resistance a needle (connected to a load cell) encounters over time. This process provides...
24 June 2024 7,534 5 View
I have been trying to grow Nicotiana benthamiana via tissue culture. I used ethanol and sodium hypochlorite with different concentrations and time duration for seeds sterilization and lastly...
18 June 2024 1,756 3 View