What does R^2 value mean?

Hello, -So I have a set of bacterial communities extraced from rhizospheric soil in both saline and control environments for two different cultivars of plants, one is tolerant to salinity and the...

07 May 2020 5,750 4 View

What does volume distribution mean for zeta sizer?

If I have a sample of 1 mL containing nanoparticles, and the graph (of volume distribution) identifies two peaks: 100 nm at 70% 200 nm at 30% Does that mean that 100 nm particles occupy 70% of the...

21 July 2019 4,419 4 View

I ran a non-trageted HPLC, because I have no standards to compare with. Can I know the compounds in a certain retention time and wavelength? How?

I ran a non-trageted HPLC, because I have no standards to compare with. Can I know the compounds in a certain retention time and wavelength? How?

26 April 2018 1,058 6 View

I ran a non-trageted HPLC, because I have no standards to compare with. Can I know the compounds in a certain retention time and wavelength? How?

I ran a non-trageted HPLC, because I have no standards to compare with. Can I know the compounds in a certain retention time and wavelength? How?

03 April 2018 7,213 7 View

How do I evaluate the QRS peaks detector?

14 September 2015 2,574 15 View

How do I evaluate the QRS peaks detector?

08 September 2015 2,587 17 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View