I am planning to express a protein from S. cerevisiae using the pYES2 plasmid that has the GAL1 inducible promoter. The protocol provided by Invitrogen says to grow cells in raffinose-containing medium before induction with galactose. However, I have read that sucrose can also be used instead of raffinose. Does anybody have experience with the system and can confirm this? As I may have to use large amounts of culture, I am looking for a cheaper alternative to raffinose.
Evelina Tutucci
Hi Tom,
you can grow your cells in rich media containing 3% glycerol, 2% lactic acid, and 0.05% glucose. This is enough to de-repress the GAL promoter. This is definitely cheaper than raffinose.
Evelina
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