Hi Benjamin

All of our research focusses on sediments, however, we do get a large number number of zooplankton in our samples, especially the deep-sea sediments due to pelagic snow. The primers we have been using work very well and will work with most zooplankton taxa, however, crustacea are notoriously difficult, so you may need to use an additional primer set there. I hope this helps. Are there any specific groups you are interested in?

Cheers

Anthony

Dear Dr Chariton,

Thanks a lot for your response.

This is very interesting for me.

I would like study what are the changes in genetic diversity of lake zooplankton communities (i.e., particularly, cladoceran, copepod and rotifer) in response to environmental forcings through a paleogenetic approach based on eDNA.

Best regards,

Benjamin

Hi there! Look I have the idea of studying lakes, different lakes with different environmental histories (natural + anthrop.), considering their pelagic biota an then studying the biota's responses(physiological to molecular and functional) under simulations of future enviromental conditions (°C, pH, etc). I think should be interesting to connect these observations with current and previous genetic diversity of lakes.

Dear Mr Aguilera,

You are right, I think also that retrospective approach could be interesting to forecasting study. Indeed, know the past and actual ecological trajectory of lakes could enhance our forecasting of response of lakes in the futur.

How do you follow biota's responses (physiological, molecular and functional), by which methods?

What is your observation scale, individual, population, community?

You say 'under simulation', therefore you work through experimental or field (lake) approach.

If field approach, what types of lakes do you study?

Best regards,

Benjamin

Hi Victor and Benjamin

I agree, the retrospective stuff is extremely interesting. We are currently dabbling in it, I shall keep you posted on how things pan out.

Cheers

Anthony

well among others, I estimate feeding, reproduction, respiration, size, protein profiles, protein synthesis. These estimates are based on individuals although most of them describe also population dynamic. All this job has been carried out in ocean ecosystems but I am interested on to use these approaches to study lakes, lakes in southern portion of Chile.