I am using Proteinase K powder from Promega for fungal DNA extraction. Can it be reconstituted in nuclease free water instead of Tris-HCl buffer and CaCl2
We used hexane and ethyl acetate to prepare dried whole plant extract of Eclipta prostrata. In its phytochemical screening, flavonoids were present in hexane extract but, it was absent in ethyl...
07 August 2020 8,202 3 View
When we conduct different screening tests to identify phytochemicals in plant extracts, we use more than one solvent. What are the criteria or how do we select the best solvent rather than using...
07 August 2020 2,933 5 View
I have got a defects subtracted image using python and openCV. Now I want to send a digital signal to an actuator to take necessary actions when a defect is detected. How can I do that? I wish to...
31 July 2018 8,067 3 View
CVA is more likely to get early in the morning and in winter. Bronchial asthma may aggravate at night. Some psychological diseases worsen on fullmoon days. Why is that?
01 January 1970 8,179 2 View
Covid 19 pandemic taught many lessons to the mankind. Online teaching were encouraged in every stage of education. Delivering online teachig was successful to great extent in university education....
01 January 1970 3,748 35 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...
03 March 2021 6,223 5 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View