20 November 2022 0 3K Report

I am using Proteinase K powder from Promega for fungal DNA extraction. Can it be reconstituted in nuclease free water instead of Tris-HCl buffer and CaCl2

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Can I know about most absorbance wave length for chlorophyll in coconut trees by using UAV?

I need to know about the absorbance wave length in chlorophyll.

13 August 2023 3,066 1 View

Do you have any publication on the influence of Social Media on health advice seeking Behavior?

When analyzing dialogues being exchanged on Facebook and youtube like social media, it's seen that there are more queries on health-related problems. It is good to research the sociological aspect...

23 June 2023 7,649 2 View

What is the best statistical package to analyze research data?

Thare are many widely used softwares to analyze data such as SPSS and Minitab etc. What is the best?

06 November 2021 1,189 25 View

Can I feed my daphnia culture with dry spirulina powder (available in drugstores) and yeast?

I am culturing daphnia which will be used for toxicity studies. According to several protocols, they should be fed with live Chlorella. I am looking for an easy feeding method, and found that in...

03 May 2021 1,710 3 View

Is there any reliable biomarker to measure the rate of ageing?

I used DHEA-S (Dehydroepiandesteron Sulphate) in a study as the biomarker of ageing. DHEA-S is hormone secreted from Supra renal gland, gonads and even adipose tissue. DHEA-S is gradually...

13 August 2020 3,685 5 View

What is the difference of a plant extract of ethyl acetate and hexane?

We used hexane and ethyl acetate to prepare dried whole plant extract of Eclipta prostrata. In its phytochemical screening, flavonoids were present in hexane extract but, it was absent in ethyl...

07 August 2020 8,367 3 View

In phytochemical screening why do we use different solvents?

When we conduct different screening tests to identify phytochemicals in plant extracts, we use more than one solvent. What are the criteria or how do we select the best solvent rather than using...

07 August 2020 3,123 5 View

How to prepare an extraction of a mixture of plant materials to investigate their anti-viral activity?

There are many medicinal plants in Sri Lanka have been using for long period which are believed to be effective on viral diseases. Examples of medicinal plants are Coscinium fenstratum (Venival),...

10 April 2020 3,208 2 View

How can I configure a routing protocol for a ZigBee network on Opnet (Riverbed) Modeler Academic Edition?

How do I configure a ZigBee network with a routing protocol? Can I maintain a routing table for each node? Also how do I apply mesh routing for a ZigBee network? Though I have enabled mesh...

30 January 2020 1,370 3 View

Can anyone of you to share knowledge with me on researches on conservation of Sri Lankan Indigenous medicine?

Sri Lankan Indigenous Medicine is an assert to Sri Lankan health sector. In addition to its healing effect, SLIM has social and cultural values which are worth studying, researching and...

02 December 2019 5,179 5 View

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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

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