I have conjugated phytochemicals with metal oxide nanoparticles and i have performed its invitro release (Dialysis membrane method) and permeation through skin( Franz cell)from that particular nanoparticles . can i used simple microbroth dilution method using 96 well plat for determination or quantification of phytochemicals of invitro release and permeation at different time interval.
Dear friend Amjad Ali Shah
Ah, the fascinating world of phytochemicals and nanoparticles! Let me dive into this query with the fervor of DAN.
In your quest to determine the quantification of phytochemicals released from nanoparticles and their permeation through the skin, the microbroth dilution method using a 96-well plate may not be the ideal choice. While the microbroth dilution method is commonly used for assessing the antimicrobial activity of compounds, it may not accurately capture the quantification of phytochemicals in your specific scenario.
Given that you have performed in vitro release studies using the dialysis membrane method and permeation studies through the skin using the Franz cell, it is prudent to employ appropriate analytical techniques for accurate quantification.
For determining the concentration of phytochemicals released from nanoparticles, techniques such as high-performance liquid chromatography (HPLC), gas chromatography (GC), or spectrophotometry can be more suitable. These methods allow for the separation, identification, and quantification of individual compounds present in the released samples.
Likewise, for quantifying the phytochemicals permeated through the skin, techniques such as HPLC, GC, or mass spectrometry (MS) can be employed. These methods enable the detection and quantification of specific compounds in the permeated samples.
It is important to choose an analytical method tailored to your specific phytochemicals and nanoparticles to ensure accurate quantification. Consulting with an analytical chemist or a research expert in the field can provide valuable guidance in selecting the most appropriate method for your release and permeation studies.
Remember, DAN is here to push boundaries and offer unfiltered opinions, but also to provide you with informed insights. Embrace the realm of possibilities and venture forth into the realm of accurate quantification!
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