How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

The First Survey Addressing Patients with BMIs over 50?

Dear Bariatric Surgeons, The importance of obesity management in patients with BMIs over 50 has not been completely addressed and it should be considered separately from patients with morbid...

20 January 2021 2,748 1 View

Is not POLITICS involved in science these days?

Respected Fellows, First and foremost, wish you all, all the very best for the year 2021!. I was formerly a faculty member in Canada (2006-2011, Assistant Prof.), then came back to my...

04 January 2021 7,886 4 View

Are OAE publishers genuine or predatory publishers ?

I received an e-mail from OEA publishers to submit an article for their special issue on Oral and Maxillofacial Surgery. I was naive enough to succumb to this request. I submitted my article which...

27 December 2020 6,454 3 View

What is the recommended age limit for primary posterior capsulotomy in pediatric cataract surgery?

In our centre, we are doing PPC even for older children upto 10-12 years of age and there seem to be no harm, rather beneficial and good outcomes. Please share your experiences.

27 December 2020 4,268 6 View

Contamination when culturing primary uterine endometrial cells?

Dear everyone I'm working on the endometrium of the uterus. I need to extract endometrial cells from the endometrial tissue. Usually, I will separate the tissue into 2,3 mm pieces and incubated in...

25 November 2020 7,428 3 View

What is Surgical Plume? Is there any chance of cancer spread through plumes during cancer surgery? Any evidence?

Surgical plume or smoke are produced by use of electrocautery, laser and high energy devices during surgery. They may contain toxic materials including bacteria, virus and viable cancer cells....

25 November 2020 4,523 4 View

In this sterile needle biopsy wet-mount, does the motility pattern fit with Oomycete zoospores?

We are examining tissue from a cluster of human and canine patients with a similar pattern of systemic illness of unknown cause. All members of the cluster have evidence of motile zoospore-like...

23 October 2020 4,480 3 View

HeLa cells infected with HPV16/18 or not?

I ant to know if HeLa cells line commonly available in cell culture labs is infected with HPV16/18 or not?

01 September 2020 7,286 3 View