I want to use overlap extension PCR to insert a domain into my construct. However I have just realised that upstream of the region where I want to insert the domain there are 19 amino acids currently present in the construct which I would like to remove. I think it should be possible to remove these residues form the current construct when I insert the new domain by overlap extension PCR- provided the primer design takes this into account. Does anyone have any experience of trying this?

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