I am trying to purified a PCR product from gel extraction (2% agarose), for the purification I used PureLink Quick gel extraction and PCR purification combo kit (Invitrogen). And for the PCR reaction I used Go Taq Green Master mix from Promega.
The purification purpose is for sequence. After the purification I made a electrophoresis. The electrophoresis conditions: 100V, 30min, 2% agarose. I used blue/orange loading dye and GelRed for staining.
As a result I got the expected bands but I also have anomalous bands in the superior part of the gel, can these be form from de loading dye? or green master mix? will this affect the results form sequencing?