Can one use computational biology to screen detergents for stabilising a particular membrane protein?

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Mahesh Chandak

So far havent seen such people ( bench workers ).

Experimental biologist dont like/trust this strategy

Annemarie Honegger

I am not aware of anyone doing so successfully. We use the cpm assay to experimentally screen for the stability of detergent solubilized membrane proteinsArticle Microscale Fluorescent Thermal Stability Assay for Membrane Proteins

, and developed the CHESS method of high throughput screening selection for protein mutants with increased stability in detergents: https://www.bioc.uzh.ch/plueckthun/pdf/APpub0345.pdf