Can microbes or their elements regulate human gene expression? Some bacterial proteins could potentially recognize promoters in human genes.
Yes, they can. Based on the published data, for example, 463 human genes are changed during an infection with Mycobacterium tuberculosis, 366 of them were known genes and the others are unknown. 25 Of the 366 human genes affected were up-regulated and the rest were down-regulated. There are two more significant down-regulated genes, CD14 receptor, down-regulated 2.3-fold and VDR receptor, down-regulated 3.3-fold.
I heard that human genes in chronic lung diseases including TB and non infectious diseases such as asbestosis or silicosis show similar gene expression repertoires. is it due to the microbial proteins regulating expression repertoires or due to change in cellular microenvironment such as pH, nutrient starvation, reactive nitrogen species?
Hi Niyaz, I suggest you look at ways that microbes can elicit immune system evasion. I think that there are some regulatory proteins released by intracelluar pathogens that have been shown to directly effect transcriptiion regulation in the host (to subvert host killing of pathogens). Sorry I can't give you the details.
The expression repertoires of host cells definitely can be affected by microenvironment alteration. Pathogen proteins are also involved in regulation (or redirection) of the host gene expression. These understood more clearly is the pathogen effectors, most of them are translocated into the host cells to regulated host response, deletion or abolishing its transport can affect the host response. e.g. some proteins in Helicobacter pylori (Hp) pathogenicity island (cag-PAI) are used to translocate the bacterial effector protein CagA into different types of eukaryotic cells to induce the synthesis and secretion of chemokines, such as interleukin-8 (IL-8).
YES.
In our recent paper we have shown such example for P. aureginosa and its major transcription factor lasR
"Uncovering effects of antibiotics on the host and microbiota using transkingdom gene networks"
the paper is available here at researchgate, see link below:
https://www.researchgate.net/publication/271213616_Uncovering_effects_of_antibiotics_on_the_host_and_microbiota_using_transkingdom_gene_networks
Article Uncovering effects of antibiotics on the host and microbiota...
Congratulations, Dr Morgun for such an elegant and exhaustive analysis.
Please let me know if the following reference/site is helpful to you:
18.1 Bacteria often respond to environmental change by regulating ...
www2.centralcatholichs.com/.../Campbell%20GE%20gene%20expression%20notes%...r
Orchestrating proper gene expression by all cells is crucial to the functions of life. environmental change by regulating transcription. Bacterial cells that can ...
Site Reference:
http://www2.centralcatholichs.com/Bio1site/DNA%20history%20and%20central%20dogma/Campbell%20GE%20gene%20expression%20notes%201.PDF
Dennis
Dennis Mazur
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