Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

Why build a Leaning Tower of Lire?

What are the lessons to be learned from building a leaning tower of Lire?

03 January 2021 7,364 4 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

What are the benefits of applying Blockchain within any industry?

Is blockchain only for cryptocurrency? Thanks in advance.

28 November 2020 5,086 11 View

Is my Chi-square test valid?

18 November 2020 3,033 2 View

Confused primer dimer or something else?

Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...

17 November 2020 1,913 5 View

Why is the corporate world and academia deluged with phenomenon of the "Peter Principle"?

Sorry, I have witnessed the Peter Principle running rampant in corporations and running twice as rampant in the academic world. Please explain.

11 November 2020 8,240 2 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

Complex wave form in ANSYS?

Is there a way to input wave speed in a complex form on ANSYS? The wave speed I need to assign a material is 325(1+0.11i) however I have been unable to do this thus far. Failing that, is there...

27 February 2021 5,969 1 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

What are difference between laser diodes, simple light emitting diodes and power light emitting diodes when operated in the visible spectrum ?

I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...

22 February 2021 4,240 4 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View