Has anyone tried using addgene LentiCrisprV2 (Plasmid # 52961) to knockout a mouse gene? The cells were resistant to puro after infection, however, none of my gRNAs can knockout the gene.
Yes lenticrispr v2 is just all about mammalian expression of cas 9 and sgRNA scafford of clone method,species,types,series and gene mutation.
A knockout mouse is a laboratory mouse in which a researchers have inactivated ,or "knocked out",an existing gene by replacing it or disrupting it with an artificial piece of DNA.
They are important animals for studying the role of genes which have been sequenced but whose functions have not determined .By causing a specific gene to be inactive in the mouse and observing any differences from normal behaviour or physiology,researchers can
inter its probable function mice are currently the lab animals species most closely related to humans for which the knockout technique can easily be applied.they are widely used in knockout experiments especially those investigating genetic questions that relate to human physiology .Gene knockout in rats is much harder and has only been possible since 2003.
This first recorded knockout mouse was created by Marior Capecchi,Martin Evans,and oliver smithies in 1959,for which they can awarded the 2007 Nobel prize in physiology or medicine .Aspects of the technology for generating knockout mice,and the mice themselves have been patented in many countries by private companies.
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