How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Loss of biodiversity its impact on terrestrial ecosystem?

I need research papers with problem statement as well as research questions in respect of the above mentioned topics

04 February 2021 2,240 15 View

Where can I find data sets containing the average mass of terrestrial predators and their prey?

For my final year thesis I require data on the average body mass of terrestrial predators and their prey. I have come across many papers which have used such data sets however, the data is not...

04 February 2021 8,348 3 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

Hi, i'm trying to synthesis Graphene oxide without (NaNO3), how ever at the end of the synthesis it just black dusty solution, any idea?

here is step from journal. 1. add 3g graphite powder in 70ml H2SO4. in ice bath and constant stirring. 2. add 9g KMnO4, after a while it become green 3. heat it to 40celcius for 30minutes, it...

22 November 2020 9,622 3 View

Why the protein is not secreted to the medium?

Dear all, Why is a protein, which is expressed in HEK293 cells, not excreted to the medium? The protein remains soluble into the cell. Which could be the reasons? Thanks for your time! Best...

04 November 2020 4,398 8 View

Antibiotics removal efficiency in WWTP and Fexcreta?

I am calculating the PEC for selected antibiotic compounds and am having problems finding the readily-available online data about the Fexcreta value for my target compounds (Since the PEC...

02 November 2020 7,675 6 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

What is Dopamine?How it affects human behaviour &directly connected to neuro-psychological disorders?

We all known Dopamine is a happy/joyous hormone secrets from brain.How it changes the over all human behaviour & responsible for the well being of humanbeing. Regards, Md Osim

02 October 2020 582 4 View

What solvents can be used to dilute the Lepidopteran defence droplets (scoli glandular secretions from lepidopteran larvae)?

I am trying to analyse the chemical profile of defensive droplets from the lepidopteran larvae (T Trypanophora semihyalina ). when i collected and tried to dilute in the hexane and acetone the...

23 September 2020 2,994 3 View