Is it possible to sequence the RNA after elution from denaturing PAGE ?

I am at the certain stage where it would be better to know the sequence of my RNA of interest. I have the question about sequencing of the PAGE purified RNA. I am thinking to prepare the cDNA of...

04 May 2017 5,159 3 View

RNA degradation problem, no 18s/28s peaks?

I am trying to extract RNA from primary culture using Trizol. But after checking on the bioanalyzer I obtained very low RIN value (3.4) and the electropherogram showed no peaks (only the marker...

10 April 2017 2,777 3 View

Anyone can tell me how to look for minisatellites in genome of an organism?

anyone can tell me how to look for minisatellites in genome of an organism.

11 January 2017 630 4 View

How to formulate phytoconstituent terpenoid in drug dosage form?

Hi All My plant of interest contain terpenoids,I request your kind suggestions in formulating terpenoids as dosage form and any possibility in formulation in nanoscience

01 September 2016 1,802 1 View

Can someone please explain how does polysome profiling data reveal the active translational state of mRNas?

I am basically interested in understanding the graph obtained after ribosome profiling and how can you know from the graph in which translational state are the mRNAs?

01 August 2016 4,482 3 View

Synthesis /Semisynthesis of natural product ? Design of synthesis procedure for natural product

Hi All I want to test my isolated phytoconstituent for some biological activity( i.e antidiabetic or anticancer).How to screen individual phytoconstituent or the synthetic analog is potent?. How...

26 April 2016 9,936 3 View

Can we study the anticancer activity of two phytoconstituents (in combination) to treat inflammation/cancer in cell lines/animal models ?

Hi All I am planning to study the molecular mechanism that link inflammation and cancer. I request you to advice me that whether two phytoconstituents(which share same biological property) can be...

25 March 2016 3,313 3 View

Does anyone have a Nav1.6, 1.7, Nav1.8 and nav1.9 plasmid to express in HEK cells?

02 March 2016 9,114 1 View

What is the standardized method to prepare DNS reagent?

Please suggest me a standardized method to prepare DNS reagent for the determination of reducing sugar by using N acetyl D glucosamine. Thank you

06 November 2014 6,095 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View