No title

Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...

18 January 2021 4,487 4 View

What fluorescent dye can be used in fixed cells for membrane imaging?

07 January 2021 4,802 2 View

QUANTUM INFORMATION: Why a Toffoli gate can be used to obtain any permutation gate?

Hi, i am sudying some quantum computer science and I am really struggling to find an explanation to the question done above. Any kind of help would be wonderfull. Greets.

09 November 2020 4,657 3 View

How to dye the spider gland of a spider to see in fluorescence (488nm)?

I want to use a fluorescence microscope to observe in situ the venom gland of a cleared spider using a 488 nm laser. Any recommendation on what dye to use that would be particularly attached to...

14 October 2020 3,986 10 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

Why doesn't my dbRDA show all the environmental variables considered?

I am performing a db-RDA in R using the package Vegan and the function capscale. I have taken into account 24 environmental factors but the program only considerate 7 of this factors. I have 54...

26 August 2020 919 6 View

How should I present microRNA expression data from a single sample (leaves)?

Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...

30 July 2020 9,696 3 View

Additionally, to GBIF database, Where I can find global records of Aedes sp. and Anopheles sp.?

Hi I'm interested in to know where find records of Aedes sp. and Anopheles sp. I visited GBIF repository but I want to know other sources of data

10 June 2020 2,535 3 View

What is the best, easiest and fastest method to construct a bacterial genomic DNA Library? Any other alternative instead fosmid kit?

I would like to clone a big region (50kb) of DNA.

08 April 2020 5,807 5 View

Murine alpha-synuclein overexpression in PD mouse model?

I have been reading about A53T aSyn genetic PD mouse model, and I realized that 99% of all of them, are based on the expression of human alpha-synuclein (wild type or mutant) in the mouse. I have...

26 March 2020 4,022 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

How to obtain parameters to run a simulation for benzene using CHARMM forcefield in GROMACS?

I have been reading this paper Soft Matter, 2017, 13, 1904 and in this paper, they simulate benzene in GROMACS using CHARMM. They mention that they performed simulations of benzene using the...

15 February 2021 5,561 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View