Can I make two separate wounds in the same animal and test, in each wound, a different concentration of my extract at the same time? wouldn't that give me false results of the activity?
Dear Chaima Belabbes
Regarding the evaluation, you mentioned using different compound concentrations in different wounds of the same model animal. However, it depends on the direction of the overall experiment to make a decision. I suggest not doing so because it will affect the independence of the individual experiment, and it is hard to say whether there will be an interaction between the wounds, which may cause misleading results. It is recommended to arrange experiments with a single concentration of compounds for a single individual.
I hope this can help your research.
Dear respected researcher,
If your are adopting different compounds for different wounds area in same animal..
You may adopt your intervention only for local application..
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