PCR with single primer

Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...

11 March 2009 7,200 6 View

assays and data interpretation

Hi, there was recently a question about real time results and how to interpret them. I would recommend the paper: Quantification of mRNA using real-time RT-PCR, from Nolan et al., Nature...

05 February 2009 9,316 0 View

T4 RNA Ligases

Hi, I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag...

03 February 2009 8,263 1 View

Polyadenylation+RT-PCR

Hi, I have been trying to reverse transcribe total bacterial RNA by polyadenylation and oligod(T) primers... I used the polyA polymerase kit from Epicentre, cleaned up the reaction on spin...

30 November 2008 8,518 4 View

BSSE and counterpoise correction in solvent

I am confused about how to run a counterpoise-corrected calculation of BSSE in a solvent. ( Actually, I mean iefpcm,solvent=water in the Gaussian 09) Or is the solvent effect important for BSSE?

01 January 1970 2,844 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View