hello

the question is quiet unclear. could please be specific

good luck bro!!!

Hi, I have mice tissues to evaluate for different parameters for each parameter I have a different biochemical kit, like MPO,MDA, SOD etc. For each kit, the manufacturer mention to homogenize tissue in their given lysis buffer. I need to know if I homogenize tissues in PBS instead of Individual lysis buffer and then use that lysate further to individual lysis buffers of kits will that work. I m asking this, cos other wise I will have to repeat my experiments MRE than 6 times to get each set of tissue for each parameter

Hi Amber

Lysis buffers contain various components that aid in the extraction of specific components. ie if there is membrane bound proteins then it needs additional components added to the lysis buffer. Ha e you centrifuged your samples after lysis? or is it the crude extract of the tissue suspended in PBS?

Thanks Angela, No I ahve not centrifuged them, and its the crude extract of the tissue suspended in PBS. I will centrifuge once I mix the desired amount in specific lysis buffer provided by the kit... but the question is shall I risk that.... as I agree with you theat lyss buffers aid in extraction.