Hi,
I am planning on constructing some endotoxin free plasmids for in utero electroporation using the QIAGEN EndoFree plasmid maxi kit. They mention having to add isopropanol both in the buffers and by itself in some of the final steps. For people who have used this kit, would it be okay to use regular isopropanol, or should I look for some endotoxin free isopropanol? The kit doesn't supply it.
Thanks!
Any isopropanol that is labelled as molecular biology grade will be suitable for this purpose. I have never heard of isopropanol being certified as free from endotoxins.
Laura Leighton That is also what I was thinking, and looking through multiple vendor websites also showed me that this is normally not a thing. However, when I contacted customer service in QIAGEN, they said they did not recommend I use regular isopropanol.
yes, you can use the same, but it should be of molecular grade only. There is a difference in chemicals concerning molecular work. Bigger giant companies wont recommend the same but it can be used.
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